Wireshark supports capturing from multiple interfaces at once.

Wireshark, TShark, and their associated utilities now save files using the pcap-ng file format by default. (Your copy of Wireshark might still use the pcap file format if pcap-ng is disabled in your preferences.)

Decryption key management for IEEE 802.11, IPsec, and ISAKMP is easier.

OID resolution is now supported on 64-bit Windows.

TCP fast retransmissions are now indicated as an expert info note, rather than a warning, just as TCP retransmissions are.

TCP window updates are no longer colorized as "Bad TCP".

TShark's command-line options have changed. The previously undocumented -P option is now -2 option for performing a two-pass analysis; the former -S option is now the -P option for printing packets even if writing to a file, and the -S option is now used to specify a different line separator between packets.

GeoIP IPv6 databases are now supported.